Cryo-EM Image Processing Tools

If there is a tool or a feature you need, please let us know.

Validation and Analysis

  • AlignProjections (1.0) - Align projections of PDB or MRC with class averages.
  • Efficiency (cryoEF) (1.1.1) - Calculate efficiency for 3D reconstruction to determine if Euler angle coverage is evenly distributed
  • DeepEMhancer (0.13) - Non-linear post-processing of cryo-EM maps for local sharpening-like post-processing and automatic masking/denoising.
  • Local B-Factor Estimation (0.0) - Local computational methods to improve the interpretability and analysis of cryo-EM maps.
  • LocBfactor Map Enhancement (0.0) - Local computational methods to improve the interpretability and analysis of cryo-EM maps.
  • LocSpiral Map Enhancement (0.0) - Local computational methods to improve the interpretability and analysis of cryo-EM maps.
  • LocOccupancy Map Analysis (0.0) - Local computational methods to improve the interpretability and analysis of cryo-EM maps.

Protein structure prediction

  • AlphaFold2 (1.0) - Highly accurate protein structure prediction with AlphaFold
  • AlphaFold2 Multimer (1.0) - Highly accurate protein structure prediction with AlphaFold Multimer
  • ColabFold (0.0) - Fast and convenient protein structure predictions using MMseqs2 and AlphaFold
  • IgFold (0.0) - Fast and convenient protein structure predictions using IgFold

RELION

Conversion

SPHIRE

  • crYOLO (1.7.6) - Automatic particle picking using crYOLO
  • ISAC 2D classification (2.3.4) - Calculate 2D class averages using iterative stable alignment and classification (ISAC)

3D heterogeneity analysis